T9577

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): Anti-C6orf175 TFB5, Anti-Chromosome 6 open reading frame 175, Anti-GTF2H5, Anti-General transcription factor IIH, polypeptide 5, Anti-TFIIH basal transcription factor complex, TTDA subunit, Anti-TGF2H5, Anti-TTDA transcription factor IIH, 8-kd subunit
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~8 kDa
• 종 반응성: mouse, rat, human
• 포장: antibody small pack of 25 μL
• 향상된 검증: recombinant expression; Learn more about Antibody Enhanced Validation
• 기술: indirect immunofluorescence: 1:200-1:400 using paraformaldehyde-fixed HEK-293T cells, over-expressing human p8/TTD-A; western blot: 1:250-1:500 using lysates of CHO or HEK-293T over-expressing human p8/TTD-A
• UniProt 수납 번호: Q6ZYL4
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GTF2H5(404672) ; mouse ... Gtf2h5(66467) ; rat ... Gtf2h5(502227)

설명

일반 설명

8-kDa trichothiodystrophy group A (p8/TTD-A) protein is the tenth subunit of general transcription factor IIH (TGFIIH). It is present as both, bound to transcription factor IIH (TFIIH) as well as a free fraction that shuffles between the cytoplasm and nucleus. The p8/TTD-A gene is located on the human chromosome at 6q25.3.

특이성

Anti-p8/TTD-A (N-terminal) specifically recognizes human, rat, and mouse p8/TTD-A.

애플리케이션

Anti-p8/TTD-A (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

생화학적/생리학적 작용

The 8-kDa trichothiodystrophy group A (p8/TTD-A) protein is required for the stability of the transcription factor IIH (TFIIH) complex. The cells from patients with p8/TTD-A have reduced levels of TFIIH while overexpressing p8/TTD-A restored the cellular level TFIIH complex. Defects in the p8/TTD-A gene are responsible for the third group of the sun-sensitive form of trichothiodystrophy (TTD), a rare autosomal recessive disorder.

표적 설명

p8/TTD-A (N-terminal) encodes a subunit of transcription/repair factor TFIIH, which functions in gene transcription and DNA repair. This protein stimulates ERCC3/XPB ATPase activity to trigger DNA opening during DNA repair, and is implicated in regulating

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)