SRP3229

PDGF-BB from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 형태: lyophilized
• 효능: 1.0-2.0 ng/mL ED₅₀
• 분자량: 24.4 kDa
• 포장: pkg of 10 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: off-white to yellow
• UniProt 수납 번호: P31240
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... PDGFB(18591)

설명

일반 설명

PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-BB is a 24.4kDa disulfide-linked homodimer of two B chains (218 total amino acids).

애플리케이션

PDGF-BB from mouse has been used as a chemotactic factor on vascular smooth muscle cell.

생화학적/생리학적 작용

The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Heterozygous mutation in PDGFB is associated with primary familial brain calcification. It is also associated with angiogenesis for osteogenesis. PDGFB induces migration of endothelial progenitor cells and mesenchymal stem cells.

서열

MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT

물리적 형태

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable