생물학적 소스
human
재조합
expressed in CHO cells
분석
≥98% (HPLC)
≥98% (SDS-PAGE)
형태
lyophilized
분자량
40 kDa
포장
pkg of 10 μg
기술
cell culture | mammalian: suitable
불순물
<0.1 EU/μg endotoxin, tested
색상
white to off-white
UniProt 수납 번호
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
human ... IL12B(3593)
일반 설명
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Accumulating data indicate that p40 secretion precedes that of IL-12 expression. In addition, to its ability to covalently bind to p35 to form IL-12, p40 can bind to p19 to form IL-23. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.
The interleukin-12 subunit β (IL12β) gene is localized on human chromosome 5q31–33. The encoded protein is part of IL-12, which is a heterodimer. It is mainly expressed by antigen-presenting cells (APCs).
생화학적/생리학적 작용
IL-12 is a disulfide-linked heterodimeric protein (p70), composed of two subunits, p35 and p40, which are encoded by two different genes. Recombinant human IL-12 p40 is a 40.0 kDa protein containing 306 amino acid residues.
Interleukin-12 subunit β (IL12β) is an immunoregulatory cytokine. It is involved in pathogenic defense responses and is also suggested as an anticancer therapeutic agent. IL12B is responsible for polarizing naive CD4+ cells toward a TH1 (T helper) phenotype and inducing IFN-γ (interferon) secretion by T and natural killer (NK) cells. This protein also stimulates the differentiation of cytotoxic CD8+ cells and enhances the activity of NK cells. IL12B is linked with many autoimmune diseases, such as psoriasis, systemic lupus erythematosus, Crohn′s disease and ulcerative colitis.
서열
p40 Subunit: IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS
물리적 형태
Lyophilized from 1x PBS, pH 7.2.
재구성
Centrifuge the vial prior to opening. Reconstitute in 1x PBS, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Interleukin-12 Immunomodulation Delays the Onset of Lethal Peritoneal Disease of Ovarian Cancer.
Cohen CA, et al.
Journal of Interferon & Cytokine Research, 36(1), 62-73 (2016)
High Interleukin-12 Levels May Prevent an Increase in the Amount of Fungi in the Gastrointestinal Tract during the First Years of Diabetes Mellitus Type 1.
Kowalewska B, et al.
Disease Markers (2016)
Li Zhang et al.
PloS one, 10(6), e0130982-e0130982 (2015-06-24)
This study aims to determine whether the genetic polymorphisms of IL-12B gene is a susceptibility factor to Ankylosing spondylitis (AS) in mainland Han Chinese population. Eight single-nucleotide polymorphisms (SNPs) (rs10045431, rs11167764, rs3212227, rs6556412, rs6556416, rs6871626, rs6887695 and rs7709212) in the
M B Atkins et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 3(3), 409-417 (1997-03-01)
A Phase I dose escalation trial of i.v. administered recombinant human interleukin 12 (rhIL-12) was performed to determine its toxicity, maximum tolerated dose (MTD), pharmacokinetics, and biological and potential antineoplastic effects. Cohorts of four to six patients with advanced cancer
M K Gately et al.
Journal of immunology (Baltimore, Md. : 1950), 147(3), 874-882 (1991-08-01)
IL-12 is a heterodimeric cytokine that was identified on the basis of its ability to synergize with IL-2 in the induction of cytotoxic effector cells and was originally called cytotoxic lymphocyte maturation factor (CLMF). IL-12 was also found to stimulate
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