SRP2035

Topo I (Y723F) (mt Y723F) human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• 동의어(들): TOPI
• UNSPSC 코드: 12352202
• NACRES: NA.26

속성

• 생물학적 소스: human
• 재조합: expressed in insect cells
• 분석: ≥80% (SDS-PAGE)
• 형태: frozen liquid
• 분자량: ~92.4 kDa
• 포장: pkg of 5 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 1000 μg/mL
• 색상: clear colorless
• NCBI 수납 번호: NM_003286
• UniProt 수납 번호: P11387
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... TOP1(7150)

설명

생화학적/생리학적 작용

Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Tyrosine 723 was identified as an active site for the DNA binding activity of DNA topoisomerase I. The covalent intermediate of topo I and DNA complex includes nucleophilic attack by the O4-oxygen of tyrosine 723 on a phosphester linkage in the DNA. Mutation from tyrosine to phenylalanine at position 723 preferentially binds the supercoiled DNA rather than relaxed DNA in the mixture of supercoiled and relaxed DNAs. But mutation at tyr⁷²³ neither affects its kinase activity that phosphorylates splicing factors of SR protein family nor its transcription activity of class II genes in vitro.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable