SRP2018

GAL4 [(1-147) + E1A (121-223)] from Saccharomyces cerevisiae canine adenovirus

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 동의어(들): E1A
• UNSPSC 코드: 12352202
• NACRES: NA.26

속성

• 생물학적 소스: Saccharomyces cerevisiae; canine
• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 형태: frozen liquid
• 분자량: ~33 kDa
• 포장: pkg of 4 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: 100 μg/mL
• 기술: electrophoretic mobility shift assay: suitable
• 색상: colorless to clear
• NCBI 수납 번호: J04368; NM_001184062
• UniProt 수납 번호: P04386
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: Saccharomyces cerevisiae ... GAL4(855828)

설명

생화학적/생리학적 작용

The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable