모든 사진(1)

Key Documents

COO/COA

SRP2007

TFIIF (RAP74 subunit) human

Name: TFIIF (RAP74 subunit) human
Brand: SIGMA

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

동의어(들):

BTF4, RAP74, TF2F1

로그인조직 및 계약 가격 보기

모든 사진(1)

About This Item

UNSPSC 코드:

12352202

NACRES:

NA.26

생물학적 소스

human

재조합

expressed in E. coli

분석

≥80% (SDS-PAGE)

형태

frozen liquid

분자량

~59 kDa

포장

pkg of 10 μg

저장 조건

avoid repeated freeze/thaw cycles

농도

200 μg/mL

기술

western blot: suitable

색상

clear colorless

NCBI 수납 번호

NM_002096

UniProt 수납 번호

P35269

배송 상태

dry ice

저장 온도

−70°C

유전자 정보

human ... GTF2F1(2962)

생화학적/생리학적 작용

The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP30) subunits that form a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. In addition to its role in transcription initiation, TFIIF can increase the specificity and efficiency of RNA polymerase II transcription, and can especially increase the rate of transcription elongation.

물리적 형태

Clear and colorless frozen liquid solution

제조 메모

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.

O Flores et al.

The Journal of biological chemistry, 264(15), 8913-8921 (1989-05-25)

The purification and characterization of transcription factor IIF (TFIIF), a factor required for transcription by the RNA polymerase II machinery, is described. TFIIF was isolated from the previously described IIE protein fraction. TFIIF enters into the transcription cycle via a

Isolation of three proteins that bind to mammalian RNA polymerase II.

M Sopta et al.

The Journal of biological chemistry, 260(18), 10353-10360 (1985-08-25)

We have used affinity chromatography on columns containing immobilized calf thymus RNA polymerase II to isolate three phosphoproteins (RAP72, RAP38, and RAP30) that bind directly to RNA polymerase II. All could be isolated from cell nuclei, and all three could

Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II.