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Merck
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문서

SH1031

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, Lentiviral Particles

Ion Channels

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About This Item

UNSPSC 코드:
41106609
NACRES:
NA.51

제품 라인

MISSION®

저장 온도

−70°C

유전자 정보

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

Lentiviral particles (representational, plate titer) are provided in 96-well plates that are barcoded for simple identification. Aliquots of 4 x 50μl are provided to prevent the need for excessive freeze/thaw, and maximize functional viral particles. Each gene family set is representationally titered (10% of clones). Fully titered sets are also available, for more information inquire at RNAi@sial.com. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

기타 정보

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 278, Number of Clones: 1456
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

법적 정보

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Declan A Doyle
Molecular membrane biology, 21(4), 221-225 (2004-09-17)
Water is probably the most important molecule in biology. It solvates molecules, all biochemical reactions occur in it and it is a major driving force in protein folding. Phospholipid membranes separate different water environments, but connections do exist between the
Stephen C Cannon
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 4(2), 174-183 (2007-03-31)
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R Zufferey et al.
Nature biotechnology, 15(9), 871-875 (1997-11-05)
Retroviral vectors derived from lentiviruses such as HIV-1 are promising tools for human gene therapy because they mediate the in vivo delivery and long-term expression of transgenes in nondividing tissues. We describe an HIV vector system in which the virulence
Sheila A Stewart et al.
RNA (New York, N.Y.), 9(4), 493-501 (2003-03-22)
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis elegans, but similar techniques have proven difficult to apply to mammalian systems. Although manipulation of the murine
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Hypochlorous acid (HOCl) is potentially an important source of cellular oxidative stress. Human HOCl exposure can occur from chlorine gas inhalation or from endogenous sources of HOCl, such as respiratory burst by phagocytes. Transcription factor Nrf2 is a key regulator

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