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Merck
모든 사진(13)

주요 문서

SAB4503817

Sigma-Aldrich

Anti-phospho-eEF2 (pThr56) antibody produced in rabbit

affinity isolated antibody

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rabbit

결합

unconjugated

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

양식

buffered aqueous solution

분자량

antigen 95 kDa

종 반응성

rat, human, mouse

농도

~1 mg/mL

기술

ELISA: 1:10000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

저장 온도

−20°C

유전자 정보

human ... EEF2(1938)

일반 설명

Eukaryotic elongation factor 2 (eEF2) is a cytoplasmic protein encoded by the gene mapped to human chromosome 19.

면역원

The antiserum was produced against synthesized peptide derived from human eEF2 around the phosphorylation site of Thr56.

Immunogen Range: 31-80

생화학적/생리학적 작용

Eukaryotic elongation factor 2 (eEF2) plays a vital role in the elongation of polypeptide chain via catalyzing the translocation of the two tRNAs and the mRNA after peptidyl transfer on the 80S ribosome. eEF2 is highly expressed in various human cancers such as lung, esophageal, pancreatic, breast and prostate cancers, head and neck squamous cell carcinoma (HNSCC), glioblastoma multiforme and non-Hodgkins lymphoma (NHL). eEF2 is used as a potential marker for the diagnosis of non-small cell lung cancer (NSCLC). eEF2 activity is inhibited by the eukaryotic elongation factor-2 kinase (eEF2K), which is essential for the transmission of growth and stress signals to protein synthesis. eEF2 functions as a biochemical sensor in neuronal dendrites that links miniature synaptic events to local translational inactivation. eEF2 is also involved in nuclear deformation and aneuploidy.

특징 및 장점

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

물리적 형태

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

Michael A Sutton et al.
Neuron, 55(4), 648-661 (2007-08-19)
Activity-dependent regulation of dendritic protein synthesis is critical for enduring changes in synaptic function, but how the unique features of distinct activity patterns are decoded by the dendritic translation machinery remains poorly understood. Here, we identify eukaryotic elongation factor-2 (eEF2)
R Jørgensen et al.
Biochemical Society transactions, 34(Pt 1), 1-6 (2005-10-26)
eEF2 (eukaryotic elongation factor 2) occupies an essential role in protein synthesis where it catalyses the translocation of the two tRNAs and the mRNA after peptidyl transfer on the 80 S ribosome. Recent crystal structures of eEF2 and the cryo-electron
Hong-Gang Sun et al.
Asian Pacific journal of cancer prevention : APJCP, 14(11), 6533-6535 (2014-01-01)
The purpose of this study was to evaluate a new type of tumor biomarker, eukaryotic elongation factor 2 (eEF2), in serum for the early diagnosis, confirmative diagnosis as well as assessment of treatment of non-small cell lung cancer (NSCLC). 130
Qi Yao et al.
The Journal of biological chemistry, 289(18), 12666-12678 (2014-03-22)
Protein-tyrosine kinase C-terminal Src kinase (Csk) was originally purified as a kinase for phosphorylating Src and other Src family kinases. The phosphorylation of a C-terminal tyrosine residue of Src family kinases suppresses their kinase activity. Therefore, most physiological studies regarding
Y Kaneda et al.
Proceedings of the National Academy of Sciences of the United States of America, 81(10), 3158-3162 (1984-05-01)
Elongation factor 2 (EF-2), polypeptidyl -tRNA translocase, is an essential factor for protein synthesis in eukaryotic cells and Archebacteria . We isolated diphtheria toxin-resistant human primary embryo cells that contain EF-2 that cannot be ADP-ribosylated by diphtheria toxin and Pseudomonas

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