생물학적 소스
rabbit
Quality Level
결합
unconjugated
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
형태
buffered aqueous solution
분자량
antigen 38 kDa
종 반응성
rat, mouse, human
농도
~1 mg/mL
기술
ELISA: 1:20000
immunofluorescence: 1:100-1:500
western blot: 1:500-1:1000
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... SAE1(10055)
관련 카테고리
일반 설명
E1 ubiquitin-activating protein AOS1 also known as SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1) is encoded by the gene mapped to human chromosome 19. Human ASO1 is expressed throughout nuclei apart from nucleoli.The protein expression is elevated in S phase and decreased in G2 phase of cell cycle.
면역원
The antiserum was produced against synthesized peptide derived from human AOS1.
Immunogen Range: 221-270
Immunogen Range: 221-270
생화학적/생리학적 작용
SAE1 (SUMOs (small ubiquitin-like modifiers) 1 activating enzyme subunit 1)/ AOS1 transcription is induced by Myc via direct binding to canonical E-Boxes sequences present near the SAE1 transcription start site. SAE1 and SAE2/ UBA2 complex plays a crucial role in the tumorigenicity of Myc-dependent cancer cells in vitro and in vivo. SAE1/SAE2 heterodimer complex catalyzes the conjugation of SUMO-1 to a variety of cellular substrates via ATP-dependent reaction, where the first step involves C-terminal adenylation of glycine residues of SUMO-1 and second step involves thioester bond formation between C-terminal glycine of SUMO-1 and a cysteine residue in SAE2.
특징 및 장점
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
물리적 형태
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Polymeric chains of SUMO-2 and SUMO-3 are conjugated to protein substrates by SAE1/SAE2 and Ubc9.
Tatham MH, et al.
The Journal of Biological Chemistry, 276(38), 35368-35374 (2001)
SUMO-activating SAE1 transcription is positively regulated by Myc.
Amente S, et al.
American Journal of Cancer Research, 2(3), 330-334 (2012)
Expression and regulation of the mammalian SUMO-1 E1 enzyme.
Azuma Y, et al.
Faseb Journal, 15(10), 1825-1827 (2001)
Robert Coukos et al.
eLife, 10 (2021-08-21)
The trafficking of specific protein cohorts to correct subcellular locations at correct times is essential for every signaling and regulatory process in biology. Gene perturbation screens could provide a powerful approach to probe the molecular mechanisms of protein trafficking, but
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.