SAB4200839

Anti-Acetylcholinesterase (AChE) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): AChE
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 농도: ~1 mg/mL
• 기술: immunoblotting: 1:1000-1:2000 using whole extracts of Jurkat cells.; immunofluorescence: 1:1000-1:20000 using Human A431
• UniProt 수납 번호: P22303
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ACHE(43)

설명

일반 설명

Acetylcholinesterase (AChE) is a member of the a/ß-hydrolase-fold proteins superfamily which is composed of structurally related proteins with a great diversity in their catalytic, recognition, adhesion and chaperone functions. 3D structure studies of a/ß-hydrolase reviled a variety of folding patterns suggesting associations through oligomerization dependent functions.1 AChE is a tissue specific serine hydrolase expressed in several variants at neuromuscular junctions (NMJs)2, cholinergic brain synapses2-3 or erythrocyte (Red Blood Cells- RBC)4.

특이성

Anti-Acetylcholinesterase (AChE) antibody specifically recognizes human AChE.

면역원

human Acetylcholinesterase, recombinant, expressed in HEK 293 cells

애플리케이션

The antibody may be used in various immunochemical techniques including Immunoblotting Immunofluorescence and ELISA. Detection of the AChE band by Immunoblotting is specifically inhibited by the immunogen.

생화학적/생리학적 작용

In the synaptic cleft, AChE main function is to ensure rapid hydrolysis of acetylcholine (ACh) released from motor nerve ending into choline and acetic acid thus preventing postsynaptic muscle ACh receptors (AChR) hyperactivation and termination of the signal transmission.2-3,5AChE and Butyrylcholinesterase (BChE) inhibitory drugs or peptides are being used in several neurodegenerative diseases including Alzheimer′s disease and pathological muscle weakness such as Myasthenia Gravis. These AChE inhibitors increase synaptic ACh levels and hence improve the cholinergic function in the brain.2-3,5-6 Erythrocytes AChE (AChE-H) is a membrane encored AChE splicing variant found in RBC, it belongs to the glycosylphosphatidylinositol (GPI) protein family and carries the Yta blood group antigen. Erythrocytes AChE can be found in plasma circulating vesicles originated from aged erythrocytes, and therefore is considered as a biomarker for RBC membrane integrity. Furthermore, Erythrocytes AChE activity is also a marker for inflammation and other diseases including essential hypertension, glaucoma, ALS, neurotoxicity, and Hirschsprung′s disease.4,7-8

물리적 형태

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

저장 및 안정성

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable