SAB4200547

Anti-IDH1 (R132S) antibody, Mouse monoclonal

clone SMab-1, purified from hybridoma cell culture

• 동의어(들): Monoclonal Anti-IDCD, Monoclonal Anti-IDH, Monoclonal Anti-IDH1 (R132S) antibody produced in mouse, Monoclonal Anti-IDP, Monoclonal Anti-IDPC, Monoclonal Anti-NADP(+)-specific ICDH, Monoclonal Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Monoclonal Anti-Oxalosuccinate decarboxylase, Monoclonal Anti-PICD, Monoclonal Anti-isocitrate dehydrogenase 1 (NADP+), soluble, NADP-dependent isocitrate dehydrogenase, peroxisomal
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: SMab-1, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~43 kDa
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: immunoblotting: 0.25-0.5 μg/mL using using total cell extracts of HEK-293T cells overexpressing IDH1^R132S
• UniProt 수납 번호: O75874
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: mutation (Arg132Ser)
• 유전자 정보: human ... IDH1(3417)

설명

일반 설명

Monoclonal Anti-IDH1 (R132S) (mouse IgG1 isotype) is derived from the hybridoma SMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized by a peptide. Isocitrate dehydrogenase 1 (IDH1) is a homodimeric, nicotinamide adenine dinucleotide phosphate (NADP)-specific enzyme. It is localized in the peroxisomes and cytoplasm. IDH1 is mapped on the human chromosome at 2q34.

특이성

Monoclonal Anti- IDH1 (R132S) recognizes only human R132S mutated IDH1 and does not cross react with others.

면역원

immunized a peptide corresponding to the mutation R132S of human IDH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

애플리케이션

Monoclonal Anti-IDH1 (R132S) antibody produced in mouse may be used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

생화학적/생리학적 작용

Isocitrate dehydrogenase (IDH) plays an important role in catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate (αKG). Mutations in this gene are associated with several cancers, such as glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutation in IDH1 specific to Arg¹³² results in the formation of 2-hydroxyglutarate (2HG) instead of αKG. 2HG hinders DNA and histone methylation resulting in alteration of gene transcription.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable