SAB4200413

Monoclonal Anti-LIN28 antibody produced in mouse

clone L28.5, tissue culture supernatant

• 동의어(들): Monoclonal Anti-CSDD1, Monoclonal Anti-LIN28A, Monoclonal Anti-ZCCHC1
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: tissue culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: L28.5, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~28 kDa
• 종 반응성: mouse, human
• 기술: western blot: 1:8,000-1:16,000 using extracts of Ntera-2 cells.
• 동형: IgM
• UniProt 수납 번호: Q9H9Z2
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... LIN28A(79727) ; mouse ... Lin28a(83557)

설명

일반 설명

LIN28 belongs to a family of RNA-binding proteins that are mainly expressed in embryonic stem cells.

면역원

synthetic peptide corresponding to a sequence at the C-terminus of human LIN28A. The isotype is determined using a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

애플리케이션

Monoclonal Anti-LIN28 antibody produced in mouse is suitable for immunoblotting at a working dilution of 1:8000 to 1:16000. For immunofluorescence, a dilution of 1:1500 to 1:3000 is recommended.

Monoclonal Anti-LIN28 antibody produced in mouse specifically recognizes C-terminus of human LIN28A.

생화학적/생리학적 작용

The structure of LIN28 includes RNA-binding domains, an N-terminal cold-shock domain and a C-terminal Zn-knuckle domain. It inhibits the biogenesis of let-7 miRNA and modulates the translation of target mRNA. The expression of LIN28 is linked to the cellular processes of development, differentiation, oncogenesis and glucose metabolism. It is also linked to the resistance of breast cancer cells to radiation effects.

물리적 형태

Solution containing 15 mM sodium azide.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable