S9822

Anti-Sonic Hedgehog peptide (C-terminal) antibody produced in goat

affinity isolated antibody, lyophilized powder

• 동의어(들): Anti-Dsh, Anti-Hhg1, Anti-Hx, Anti-Shh
• UNSPSC 코드: 51111800
• NACRES: NA.41

속성

• 생물학적 소스: goat
• Quality Level: 100
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: lyophilized powder
• 종 반응성: mouse
• 기술: immunohistochemistry: 15 μg/mL; western blot: 0.1-0.2 μg/mL
• UniProt 수납 번호: Q62226
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: mouse ... Shh(20423)

설명

일반 설명

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog Peptide (C-terminal) recognizes mouse Sonic Hedgehog C-terminal peptide. This antibody shows less than 5% cross-reactivity with rmDhh C-terminal peptide and less than 1% cross-reactivity with a 6X histidine-tagged rmShh N-terminal peptide (amino acids 25-198).

면역원

purified, E. coli-derived recombinant mouse 6× histidine-tagged sonic hedgehog C-terminal peptide.

애플리케이션

Anti-Sonic Hedgehog Peptide (C-terminal) can be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For immunohistochemistry, a working concentration of 15 μg/ml is recommended.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)