S6446

Anti-SMC1L1 (C-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): Anti-SMC1, Anti-SMC1 structural maintenance of chromosomes 1-like 1, Anti-SMC1α
• MDL number: MFCD03791776
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen 160 kDa
• 종 반응성: rat (predicted), human, mouse (predicted)
• 기술: immunoprecipitation (IP): 5-10 μL using HEK 293-T cell lysates; indirect immunofluorescence: 1:100-1:200 using paraformaldehyde-fixed HEK 293-T cells; microarray: suitable; western blot: 1:500-1:1,000 using HeLa nuclear extracts
• UniProt 수납 번호: Q14683
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SMC1A(8243) ; mouse ... Smc1a(24061) ; rat ... Smc1a(63996)

설명

일반 설명

Structural maintenance of chromosomes protein 1A (SMC1A, SMC1L1, SMC1), a structural maintenance of chromosomes (SMC) protein belongs to the SMC superfamily and is an important constituent of the cohesin complex. This gene is located on human chromosome Xp11.22.

특이성

Anti SMC1L1 (also known as SMC1 and SMC1a) specifically recognizes SMC1L1.

면역원

synthetic peptide corresponding to amino acids 1210-1225 of human SMC1L1, conjugated to KLH via an N-terminal cysteine residue. The immunizing sequence is conserved in mouse and rat.

애플리케이션

Anti-SMC1L1 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

생화학적/생리학적 작용

Structural maintenance of chromosomes protein 1A (SMC1A, SMC1L1, SMC1) can be phosphorylated by Ataxia-telangiectasia, mutated (ATM) indicating a potential role for this protein in DNA repair. Thus, SMC1L1 phosphorylation appears to be the critical downstream event in the ATM-nibrin (NBS1)-breast cancer type 1 (BRCA1) pathway, which mediates cell survival and chromosomal stabilization after DNA damage. Mutations in SMC1L1 gene result in the Cornelia de Lange syndrome, a multisystem developmental disorder. It participates in chromosome dynamics, double-strand break (DSB) repair and tumorigenesis. This gene can control the cell cycle and helps to maintain the stability of genome. Suppression of SMC1A can block the proliferation and migration of hepatocellular carcinoma cells.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)