R8029

Anti-RAD17 (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-S.cerevisiae homolog of RAD24
• MDL number: MFCD08705510
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen 70 kDa (also recognizes 75 kDa)
• 종 반응성: human
• 농도: ~1 mg/mL
• 기술: immunoprecipitation (IP): suitable; indirect immunofluorescence: 5-10 μg/mL using HEK293-T cells fixed with methanol-acetone; microarray: suitable; western blot: 0.5-1.0 μg/mL using HeLa cell lysates
• UniProt 수납 번호: O75943
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... RAD17(5884)

설명

일반 설명

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component, a homolog of the Schizosaccharomyces pombe checkpoint gene, rad17 is located on human chromosome 5q13.2. This gene is abundantly expressed in the testis.

특이성

Anti RAD17 (C-terminal) specifically recognizes RAD17 (70 kDa and 75 kDa).

면역원

synthetic peptide corresponding to amino acids 623-640 of human Rad17 (isoform 2), conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is present in isoforms 1-4 of RAD17.

애플리케이션

Anti-RAD17 (C-terminal) antibody produced in rabbit may be used in:

• immunoblotting
• immunoprecipitation
• immunofluorescence

생화학적/생리학적 작용

RFC-like DNA damage sensor (RAD17)/checkpoint clamp loader component participates in DNA replication, repair and modification or chromatin remodeling. Phosphorylation of RAD17 is a critical event during checkpoint signaling in DNA-damaged cells. RAD17 has been reported in colon, breast and non-small cell lung carcinoma. It may also participate in cell cycle control.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)