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Merck
모든 사진(3)

문서

R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

동의어(들):

Transfer RNA, tRNA

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352106
NACRES:
NA.55

Grade

for molecular biology

Quality Level

유형

Type V

형태

lyophilized powder

특이 활성도

15-19 units/mg solid

저장 조건

desiccated

solubility

H2O: ~2 mg/mL, clear to hazy

저장 온도

2-8°C

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일반 설명

Wheat germ tRNA contains many different specific tRNA molecules.

애플리케이션

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

성분

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

단위 정의

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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