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Merck
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Key Documents

R6878

Sigma-Aldrich

Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

동의어(들):

Anti-phospho-Rb (pSer795)

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

결합

unconjugated

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

RB-10, monoclonal

형태

buffered aqueous solution

분자량

antigen 110 kDa

종 반응성

mouse, rat, human, bovine, hamster

농도

~2 mg/mL

기술

immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361

동형

IgG1

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

phosphorylation (pSer795)

유전자 정보

human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)

일반 설명

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

면역원

synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer795) of human retinoblastoma protein conjugated to KLH.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunofluorescence
  • fluorescence-activated cell sorting (FACS) assays
  • enzyme linked immunosorbent assay (ELISA)

생화학적/생리학적 작용

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS795 is required to inactivate Rb imposed growth suppression.

물리적 형태

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Karen J Meaburn et al.
Aging cell, 6(2), 139-153 (2007-02-06)
A number of diseases associated with specific tissue degeneration and premature aging have mutations in the nuclear envelope proteins A-type lamins or emerin. Those diseases with A-type lamin mutation are inclusively termed laminopathies. Due to various hypothetical roles of nuclear
The E2F1-3 transcription factors are essential for cellular proliferation
Wu L, et al.
Nature, 414(6862), 457-457 (2001)
Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells
Ohmine S, et al.
Oncology Reports, 39(4), 1725-1730 (2018)
Cellular mechanisms of tumour suppression by the retinoblastoma gene
Burkhart DL and Sage J
Nature Reviews. Cancer, 8(9), 671-671 (2008)
Yan Li et al.
Molecular medicine reports, 14(3), 2222-2230 (2016-07-20)
Ginsenoside Rg3 (Rg3) is one of the primary constituents isolated from ginseng, and has been found to exhibit cytotoxic effects against cancer cells. The present study aimed to investigate the effects of Rg3 on human multiple myeloma cell proliferation and

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