R2779

Renin human

BioUltra, recombinant, expressed in mammalian cells, ≥90 unit/μg protein, ≥98% (SDS-PAGE)

• 효소 위원회 번호: 3.4.23.15 (BRENDA, IUBMB)
• MDL number: MFCD00132171
• UNSPSC 코드: 12352204

속성

• 재조합: expressed in mammalian cells
• 제품 라인: BioUltra
• 분석: ≥98% (SDS-PAGE)
• 특이 활성도: ≥90 unit/μg protein
• UniProt 수납 번호: P00797
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... REN(5972)

설명

애플리케이션

Human renin is a member of the Peptidase A1 family. It catalyzes the hydrolysis of angiotensinogen to produce angiotensin I. It is used to study human renin-dependent hypertension as well as renin induced obesity .

생화학적/생리학적 작용

Renin is a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds. Renin, an aspartyl protease, cleaves angiotensinogen which results in Ang I. Ang I is converted to Ang II by the Ang-converting enzyme (ACE) . Prorenin is hydrolyzed by plasma kallekrein to produce active renin. The human renin transgene is thought to be responsible for increased appetite, obesity, and metabolic changes which are not related to angiotensin II .

물리적 특성

Renin is processed as a 406 amino acid precursor (45,057 Da) containing a 23 amino acid signal peptide and a 43 amino acid propeptide. Native active renin is a 340 amino acid glycoprotein containing three disulfide bonds.

Supplied as a solution in 50 mM Tris HCl, pH 8

단위 정의

One unit will produce one fluorescent unit from the hydrolysis of Renin Substrate I (Arg-Glu(EDANS)-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Lys(dabcyl)-Arg) at 37 °C at pH 8.0

제조 메모

Expressed in mammalian cells as prorenin. The prorenin is activated to renin using trypsin and re-purified.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)