PLN350

GenElute^™ Plasmid Miniprep Kit

sufficient for 350 purifications

• 동의어(들): GenElute^™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
• UNSPSC 코드: 41105501
• NACRES: NA.52

속성

• 사용: sufficient for 350 purifications
• 환경친화적 대안 제품 특성: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 기술: DNA purification: suitable
• 환경친화적 대안 카테고리: Aligned,
• 저장 온도: 15-25°C

설명

일반 설명

The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

애플리케이션

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.

The kit has been used for the purification of plasmid DNA of Dirofilaria repens.

특징 및 장점

• Purified plasmid DNA in less than 30 minutes
• Purify up to 15 μg of plasmid DNA
• No detectable genomic DNA or RNA contamination
• No phenol/chloroform extraction or alcohol precipitation required

기타 정보

For additional information, please see www.sigma-aldrich.com/genelutehp.

법적 정보

GenElute is a trademark of Sigma-Aldrich Co. LLC

안전성 정보

• 픽토그램: GHS02,GHS08,GHS05,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H226,H290,H302,H315,H319,H334,H336
• 예방조치 성명서: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Central nervous system
• Storage Class Code: 3 - Flammable liquids