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Merck
모든 사진(1)

문서

P7338

Sigma-Aldrich

Protease from HIV-1

recombinant, expressed in E. coli, buffered aqueous solution, suitable for cleaving HIV substrate III (Yields 2 peaks which elute before the substrate peak when analyzed by reversed phase HPLC.)

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352200

재조합

expressed in E. coli

형태

buffered aqueous solution

적합성

suitable for cleaving HIV substrate III (Yields 2 peaks which elute before the substrate peak when analyzed by reversed phase HPLC.)

배송 상태

dry ice

저장 온도

−70°C

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단위 정의

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

물리적 형태

Solution in dilute HCl, pH 1.6.

시험 성적서(COA)

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문서 라이브러리 방문

A Leuthardt et al.
FEBS letters, 326(1-3), 275-280 (1993-07-12)
The gene coding for the HIV-1 protease was cloned in an Escherichia coli expression vector adding three-histidine codons to the amino and carboxy terminus of the protease sequence. Expression of the protease from this construct led to the accumulation of
J M Louis et al.
Nature structural biology, 6(9), 868-875 (1999-08-31)
In the Gag-Pol polyprotein of HIV-1, the 99-amino acid protease is flanked at its N-terminus by a transframe region (TFR) composed of the transframe octapeptide (TFP) and 48 amino acids of the p6pol, separated by a protease cleavage site. The
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