P2494

Protein Staining and Identification Techniques

• ISBN-10: 1-881299-08-2
• ISBN-13: 978-1-881299-08-0
• UNSPSC 코드: 55101509

속성

• 출판물 정보: R. Allen and B. Budowle, ed., Eaton Publishing, 2000, 160 pp., soft cover
• 제조업체/상표: Eaton Publishing

설명

일반 설명

Staining techniques are fundamental tools for the study of proteins, but information on protein-specific staining procedures has remained scattered. This book presents a spectrum of protein staining procedures, including wool-dye, metallic, enzyme, fluorescence, and immunostaining, the latter both in situ and following transfer to membranes. Many of these stains have their origins as histochemical techniques. Finally, techniques for obtaining photographic or electronic images of gel separations are presented in a section on the preparation, archiving, and presentation of gel reproductions.

목차

Introduction
1. Protein Staining Amido Black 10B (Buffalo Black NBR); Coomassie Blue Purification: Brilliant Blue Staining, CBB R-250 and G-250, Colloidal CBB; Serva Violet; Crowle′s and Cline′s Double Stain; Procion Brilliant Blue; Fast Green FCF; Stains-All
2. Silver Staining Diamine stains: ultrathin-layer gels, thick gels; Oxidative Silver Stain; Colored Silver Stains; Rapid Silver Stain; Agarose Coomassie/Silver Stain
3. Multiple Molecular Forms of Enzymes Hydrolases: erythrocyte acid phosphatase, Esterases, dipeptidyl aminopeptidase, ATPase; Oxidoreductases: oxidases, dehydrogenases, lactic dehydrogenase, malic dehydrogenase, glucose-6-phosphate dehydrogenase; Transferases, Lyases, Isomerases, Ligases: glutamic pyruvic transaminase, phosphoglucomutase, galactokinase
4. Negative Staining Negative Gold; Negative Copper; Negative Zinc; CBB-Zinc Double Stain; Methyl Tricholoroacetate
5. Fluorescence Pre-electrophoretic Stains; Post-electrophoretic Stains; ANS Staining; MDPF/Fluorescamine; Nile Red; Direct Detection of Proteins; Sypro Dyes: Sypro Red and Sypro Orange
6. Identification Periodic Acid-Schiff stain; Conconavalin A/Horseradish Peroxidase; Con A/Coomassie Brilliant Blue R-250; Alcian Blue; Optimal Staining of Macrogels and Minigels; Lipoprotein Prestain; Affinity Immunodeletion; Enzyme Inhibitors; Sequence Identity; Pattern Simplification; Specific Lectin Affinity Electrophoresis
7. Transfer Methods Blotting; Immunoprinting; Pressure-Blotting onto Nitrocellulose Membrane; Peroxidase- and Alkaline Phosphatase-Conjugated Antibody; Chemiluminescence; Chemifluorescence; Protein Transfer Technique: digoxigenin-luminescence visualization; Autoradiography; Colloidal Metals
8. Image Handling Procedures Photography: black and white, color; Flatbed Scanners; Solid-State CCD Cameras; Densitometry and Direct Image Processing; Image Transducers; Image Processing