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Merck
모든 사진(2)

문서

MBD0018

Sigma-Aldrich

Microbial DNA standard from Bacillus subtilis

Suitable for PCR, Sequencing and NGS, 10 ng/μL

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About This Item

UNSPSC 코드:
41105500
NACRES:
NA.24

Quality Level

형태

liquid

농도

10 ng/μL

기술

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

배송 상태

ambient

저장 온도

−20°C

관련 카테고리

일반 설명

Microbial DNA standard from Bacillus subtilis (B. subtilis) is a genomic DNA microbial standard that can serve as a standard for benchmarking the performance, along the workflow of microbiomics or meta-genomics analyses. It can also be used as a tool to increase reproducibility and allow comparison of results obtained by different labs. Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Bacillus subtilis is a gram positive, aerobic bacterium, rod-shaped, endospore-forming, catalase-positive. It is traditionally considered as a soil microorganism. Bacillus subtilis is genetically well characterized and serves as a model organism for important gram-positive pathogens such as Bacillus anthracis, Staphylococcus aureus etc. B. subtilis possess an extreme adaptation ability to various environmental stress conditions by modifying its motility, uptake of exogenous DNA, biofilm formation and sporulation. Recent studies have identified and characterized B. subtilis isolates from human gastrointestinal track suggesting B. subtilis as a gut commensal. Moreover, B. subtilis presence in the gut was found to correlate with probiotic effects such as anti-diarrhea effect, anti-microbial effect, immune-stimulatory effect, competition with pathogens, normal flora growth stimulation and prevention of intestinal inflammation. B. subtilis beneficial effect on gut inflammation may also suggest it as potential probiotic treatment for inflammatory bowel diseases (IBD), including Crohn′s and ulcerative colitis. In addition, it was demonstrated that a peptide produced by B. subtilis possess antiviral effect against influenza virus.




Read here how to use our standards to ensure data integrity for your microbiome research.

애플리케이션

Suitable for Quantitative standard for PCR, Sequencing and NGS

특징 및 장점

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

물리적 형태

Liquid - The genomic DNA is provided at >=10 ng/μL concentration in TE buffer pH 8.0

기타 정보

It is recommended to avoid freeze thaw cycles of this product.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

이미 열람한 고객

Amelioration of the DSS-induced colitis in mice by pretreatment with 4, 4'-diaponeurosporene-producing Bacillus subtilis
Jing Y, et al.
Experimental and Therapeutic Medicine, 14(6), 6069-6073 (2017)
Bacillus subtilis?The Representative of Gram-Positive Bacteria
The Flagellar World, 22-23 (2014)
Subti wiki-a database for the model organism Bacillus subtilis that links pathway, interaction and expression information
Michna RH, et al.
Nucleic Acids Research, 42, D692-D698 (2013)

문서

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.