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Merck
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문서

MAK434

Sigma-Aldrich

Acetaldehyde Assay Kit

Sufficient for 100 colorimetric tests

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

Quality Level

입력

cell culture medium
tissue
serum
urine
food(s)
beverage(s)
plasma

응용 분야

agriculture
cosmetics
environmental
food and beverages

검출 방법

colorimetric

관련 질환(들)

gastrointestinal diseases

저장 온도

−20°C

일반 설명

Acetaldehyde (CH₃CHO) is a commonly found aldehyde in nature and widely used in various industries. It is a metabolic byproduct of ethanol in the liver and is toxic to the human body. However, it is rapidly converted to the less harmful acetic acid by the enzyme aldehyde dehydrogenase. Individuals deficient in aldehyde dehydrogenase experience accumulation of acetaldehyde upon alcohol consumption, leading to facial and body flushing known as alcohol flush reaction or “Asian flush syndrome.” Acetaldehyde buildup has also been linked to alcohol-related hangover effects. Despite its classification as a carcinogen, acetaldehyde is naturally present in many foods and beverages such as ripe fruit, coffee, and wine.

애플리케이션

The Acetaldehyde Assay Kit may be used for:
  • Alcohol Metabolism Research
  • Hepatology Research
  • Neurological Research

특징 및 장점

  • Simplified Process: Enjoy a hassle-free procedure that requires the addition of a single working reagent and only a 30 minute assay room temperature reaction, saving you time and effort.
  • Convenient and Sensitive: Uses only 20 µL of sample for the determination of acetaldehyde with a detection range of 2 µM to 2mM.
  • High-Throughput Compatibility: Seamlessly integrate our kit into high-throughput handling systems, ensuring efficiency and accuracy.

적합성

The kit is suitable for acetaldehyde determination in biological samples (e.g. plasma, serum, urine, tissue and culture media) and food/beverage samples (e.g. wine, coffee, and juice).

원리

The Acetaldehyde Assay Kit is based on the aldehyde dehydrogenase catalyzed oxidation of acetaldehyde with the reduction of NAD to NADH. The formed NADH subsequently reduces MTT, producing a colored formazan compound. The intensity of the end product color, measured at 565 nm, is directly proportional to the acetaldehyde concentration in the sample.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids


시험 성적서(COA)

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