M8407

Methotrexate hydrate

powder, suitable for cell culture, BioReagent

• 동의어(들): 4-Amino-10-methylfolic acid hydrate, L-4-Amino-N¹⁰-methylpteroylglutamic acid hydrate, L-Amethopterin hydrate, Antifolan hydrate, MTX hydrate, Methylaminopterin hydrate
• 실험식(Hill 표기법): C₂₀H₂₂N₈O₅ · xH₂O
• CAS Number: 133073-73-1
• Molecular Weight: 454.44 (anhydrous basis)
• MDL number: MFCD00150847
• UNSPSC 코드: 12352207
• PubChem Substance ID: 24897252
• NACRES: NA.75

속성

• product name: Methotrexate hydrate, powder, BioReagent, suitable for cell culture, ≥98% (HPLC)
• 제품 라인: BioReagent
• 분석: ≥98% (HPLC)
• 형태: powder
• 기술: cell culture | mammalian: suitable
• solubility: H₂O: insoluble
• 항생제 활성 스펙트럼: neoplastics; parasites
• 동작 모드: enzyme | inhibits
• 저장 온도: −20°C
• SMILES string: [H]O[H].CN(Cc1cnc2nc(N)nc(N)c2n1)c3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O
• InChI: 1S/C20H22N8O5.H2O/c1-28(9-11-8-23-17-15(24-11)16(21)26-20(22)27-17)12-4-2-10(3-5-12)18(31)25-13(19(32)33)6-7-14(29)30;/h2-5,8,13H,6-7,9H2,1H3,(H,25,31)(H,29,30)(H,32,33)(H4,21,22,23,26,27);1H2/t13-;/m0./s1
• InChI key: FPJYMUQSRFJSEW-ZOWNYOTGSA-N
• 유전자 정보: human ... DHFR(1719)

설명

애플리케이션

Methotrexate hydrate has been used:

• as a dihydrofolate (DHFR) inhibitor in sphere forming assay culture
• as a component of dulbecco′ modified Eagle′s medium (DMEM) for selection of transfected cells
• in viability and co-culture assays

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites.

Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.

생화학적/생리학적 작용

Methotrexate is a folic acid antagonist.

Methotrexate used to inhibit dihyrofolate reductase and select for DHFR overexpresssion.

안전성 정보

• 픽토그램: GHS06,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H301,H315,H319,H360FD
• 예방조치 성명서: P202 - P264 - P280 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges