This product is not soluble in either water or PBS. This product is tested for solubility at 1mg/mL in acetone. In addition, this product is also soluble in Chloroform at 10 mg/mL. A brief search of the literature reveals that a 1:1 mixture of DMSO and acidified isopropanol has been used successfully to dissolve this material.
M2003
MTT Formazan
powder
๋์์ด(๋ค):
1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
๋ก๊ทธ์ธ์กฐ์ง ๋ฐ ๊ณ์ฝ ๊ฐ๊ฒฉ ๋ณด๊ธฐ
ํฌ๊ธฐ ์ ํ
1 G
โฉ277,806
๋ชจ๋ ์ฌ์ง(2)
ํฌ๊ธฐ ์ ํ
๋ณด๊ธฐ ๋ณ๊ฒฝ
1 G
โฉ277,806
About This Item
์คํ์(Hill ํ๊ธฐ๋ฒ):
C18H17N5S
CAS Number:
Molecular Weight:
335.43
MDL number:
UNSPSC ์ฝ๋:
12171500
PubChem Substance ID:
NACRES:
NA.47
์ถ์ฒ ์ ํ
๋ถ์
โฅ97% (TLC)
์์
powder
์์
purple
solubility
acetone: 1ย mg/mL
์์ฉ ๋ถ์ผ
diagnostic assay manufacturing
hematology
histology
์ ์ฅ ์จ๋
room temp
SMILES string
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key
GIFKVQOPAJDJLO-SZVLODPRSA-N
์ ์ฌํ ์ ํ์ ์ฐพ์ผ์ญ๋๊น? ๋ฐฉ๋ฌธ ์ ํ ๋น๊ต ์๋ด
๊ด๋ จ ์นดํ ๊ณ ๋ฆฌ
์ํํ์ /์๋ฆฌํ์ ์์ฉ
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
๊ด๋ จ ์ ํ
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (ยฐF)
Not applicable
Flash Point (ยฐC)
Not applicable
๊ฐ์ธ ๋ณดํธ ์ฅ๋น
Eyeshields, Gloves, type N95 (US)
๊ฐ์ฅ ์ต์ ๋ฒ์ ์ค ํ๋๋ฅผ ์ ํํ์ธ์:
์ํ ์ฑ์ ์(COA)
Lot/Batch Number
์ ํฉํ ๋ฒ์ ์ ์ฐพ์ ์ ์์ผ์ ๊ฐ์?
ํน์ ๋ฒ์ ์ด ํ์ํ ๊ฒฝ์ฐ ๋กํธ ๋ฒํธ๋ ๋ฐฐ์น ๋ฒํธ๋ก ํน์ ์ธ์ฆ์๋ฅผ ์ฐพ์ ์ ์์ต๋๋ค.
์ด ์ ํ์ ์ด๋ฏธ ๊ฐ์ง๊ณ ๊ณ์ญ๋๊น?
๋ฌธ์ ๋ผ์ด๋ธ๋ฌ๋ฆฌ์์ ์ต๊ทผ์ ๊ตฌ๋งคํ ์ ํ์ ๋ํ ๋ฌธ์๋ฅผ ์ฐพ์๋ณด์ธ์.
์ด๋ฏธ ์ด๋ํ ๊ณ ๊ฐ
Apoptosis in Neurobiology, Frontiers in Neuroscience (1998)
Onofrio Zirafi et al.
Science translational medicine, 6(262), 262ra157-262ra157 (2014-11-14)
Topically applied microbicides potently inhibit HIV in vitro but have largely failed to exert protective effects in clinical trials. One possible reason for this discrepancy is that the preclinical testing of microbicides does not faithfully reflect the conditions of HIV
Sukant Garg et al.
Marine drugs, 17(6) (2019-06-15)
Fucoxanthin is commonly found in marine organisms; however, to date, it has been one of the scarcely explored natural compounds. We investigated its activities in human cancer cell culture-based viability, migration, and molecular assays, and found that it possesses strong
LncRNA GAS6-AS2 promotes bladder cancer proliferation and metastasis via GAS6-AS2/miR-298/CDK9 axis.
Xin Rui et al.
Journal of cellular and molecular medicine, 23(2), 865-876 (2018-11-06)
Long noncoding RNAs (lncRNAs) have been proved to play important roles in carcinogenesis and development of numerous cancers, but their biological functions in bladder cancer remain largely unknown. In this study, a novel lncRNA termed GAS6-AS2 were primary identified, and
DJ-1 deficiency triggers microglia sensitivity to dopamine toward a pro-inflammatory phenotype that is attenuated by rasagiline.
Trudler D
Journal of Neurochemistry, 129, 434-434 (2014)
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What is the solubility of MTT Formazan, Product M2003, in water or PBS?
1 ๋ต๋ณ-
๋์์ด ๋์์ต๋๊น?
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Is MTT formazan (M2003) used to measure cell viability? if it how to used it?
1 ๋ต๋ณ-
No. The MTT Formazan is the reduced or cleaved form of MTT and would not be suitable in determining cell viability. For viability or metabolic cell studies see product M5655.
https://www.sigmaaldrich.com/product/sigma/m5655๋์์ด ๋์์ต๋๊น?
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What is the Department of Transportation shipping information for this product?
1 ๋ต๋ณ-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
๋์์ด ๋์์ต๋๊น?
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Can MTT Formazan, Product M2003, be used in cell viability assays?
1 ๋ต๋ณ-
No.ย ย MTT or thiazolyl blue tetrazolium is used to determine the number of viable cells in well-cultures. Consider use of kit CGD1 or product M5655.
๋์์ด ๋์์ต๋๊น?
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Can MTT Formazan, Product M2003, be used to measure dehydrogenases?
1 ๋ต๋ณ-
No. MTT is converted by active mitochondrial dehydrogenases of living cells to MTT Formazan. Consider use of product M2128 or M5655.
๋์์ด ๋์์ต๋๊น?
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What is the proper wavelength for measuring MTT Formazan, Product M2003?
1 ๋ต๋ณ-
We recommend detection at 570 nm, with background subtraction at 630-690 nm in acidified isopropanol.
๋์์ด ๋์์ต๋๊น?
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