L2137

Lipopolysaccharides from Salmonella enterica serotype minnesota

purified by gel-filtration chromatography

• 동의어(들): LPS
• MDL number: MFCD00131520
• UNSPSC 코드: 12352201
• NACRES: NA.25

속성

• 생물학적 소스: Salmonella enterica (Serotype minnesota)
• Quality Level: 200
• 형태: lyophilized powder
• 정제법: gel-filtration chromatography
• 불순물: <1% Protein
• 색상: white
• solubility: water: 4.90-5.10 mg/mL, cloudy to hazy, colorless
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

This product is extracted from Salmonella Minnesota and purified by gel filtration. The source strain is ATCC 9700. Mechanisms of complement activation by LPS of both rough and smooth strains of Salmonella Minnesota were studied by immunoassay of the activation products.

애플리케이션

• TRPV4 is not the molecular sensor for bacterial lipopolysaccharides-induced calcium signaling: This study explores the signaling pathways activated by lipopolysaccharides from Salmonella enterica serotype Minnesota, demonstrating that TRPV4 is not involved, advancing understanding of immune responses to bacterial endotoxins (Wang et al., 2023).

생화학적/생리학적 작용

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

제조 메모

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

기타 정보

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable