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문서

GE28-4082-56

HiTrap® MabSelect

Cytiva 28-4082-56, pack of 5 × 5 mL

동의어(들):

HiTrap MabSelect, MabSelect column

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

recombinant protein A (E. coli)

포장

pack of 5 × 5 mL

제조업체/상표

Cytiva 28-4082-56

저장 조건

(20% Ehtanol)
(20% Ethanol)

파라미터

42 psi

베드 크기

16 mm × 25 mm

베드 용적

5 mL

컬럼 I.D.

16 mm

기질

highly cross-linked agarose

평균 부품 크기

85 μm (d₅₀v is the median particle size of the cumulative volume distribution)

cleaning

2-12

working range

3-10

용량

≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)

적합성

suitable for bioprocess medium

저장 온도

2-8°C

관련 카테고리

일반 설명

Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 20 cm.
HiTrap® MabSelect columns are prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

애플리케이션

Lab Purification using the MabSelect Protein A tag for capture of antibodies.

특징 및 장점

  • HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
  • Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
  • Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva
MabSelect is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

프로토콜

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

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