추천 제품
ligand
recombinant protein A (E. coli)
포장
pack of 5 × 5 mL
제조업체/상표
Cytiva 28-4082-56
저장 조건
(20% Ehtanol)
(20% Ethanol)
파라미터
42 psi
베드 크기
16 mm × 25 mm
베드 용적
5 mL
컬럼 I.D.
16 mm
기질
highly cross-linked agarose
평균 부품 크기
85 μm (d₅₀v is the median particle size of the cumulative volume distribution)
cleaning
2-12
working range
3-10
용량
≥30 mg binding capacity (humanlgG/ml at 2.4 min residence time)
적합성
suitable for bioprocess medium
저장 온도
2-8°C
관련 카테고리
일반 설명
애플리케이션
특징 및 장점
- HiTrap® columns prepacked with MabSelect, a Bioprocess medium for capuring of Mabs from large sample volumes. • The specificity of binding to the Fc region of IgG is similar to that of native protein A, which allows one-step purification.
- Oriented coupling of recombinant Protein A to the matrix via an engineered C-terminal cysteine enhances IgG binding capacity.
- Fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
저장 및 안정성
분석 메모
기타 정보
법적 정보
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
프로토콜
Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.
This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.