형태
solid
특징
wetted part: no
포장
pack of 12 strips
제조업체/상표
Cytiva 17-6001-95
색상
Colorless
solubility
cold water: soluble (Insoluble in cold and hot water)
배송 상태
dry ice
저장 온도
−20°C
일반 설명
Immobiline® DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application.
애플리케이션
Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and Protein loading capacity available, which results in well separated spots with high Protein content after the second-dimension separation.
The pH 3–11 NL strip covers most Proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the Proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of Protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
The pH 3–11 NL strip covers most Proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the Proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of Protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
특징 및 장점
- Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
- Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
- Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total Protein distribution.
- For more detailed studies or when using greater Protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
- Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
- Use matching IPG buffers for optimized running conditions.
- Use IPG strips directly after reswelling without further manipulation.
- Barcoded strips for convenient sample and batch tracking.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Ettan is a trademark of Cytiva
Immobiline is a registered trademark of Cytiva
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
프로토콜
This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.
관련 콘텐츠
This page shows how to apply equilibrated Immobiline DryStrip gels
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