E6157

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-EIF-2B, Anti-EIF2B-beta, Anti-EIF2BB, Anti-Eukaryotic translation initiation factor 2B, beta, Anti-Eukaryotic translation initiation factor 2B, subunit 2
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~39 kDa
• 종 반응성: rat, mouse, human
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): 5-10 μg using K562 cell lysates; western blot: 1-2 μg/mL using K562 cell lysates
• UniProt 수납 번호: P49770
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EIF2B2(8892) ; mouse ... Eif2b2(217715) ; rat ... Eif2b2(84005)

설명

일반 설명

Translation initiation factor eIF-2B subunit beta, eIF2B β is a component of the regulatory subcomplex of eIF2B along with the eIF2Bα and -δ subunits. eIF2Bβ gene is mapped to human chromosome 14q24.3.

특이성

Anti-eIF2Bβ (C-terminal) specifically recognizes human, mouse, and rat eIF2Bβ.

애플리케이션

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μg using K562 cell lysates and western blotting at a concentration of 1-2μg/mL using K562 cell lysates.

Anti-eIF2Bβ (C-terminal) antibody produced in rabbit may be used in immunoblotting.

생화학적/생리학적 작용

The eIF2Bα,-β, and -δ subunits downregulate eIF2B activity in response to the phosphorylation of eIF2 on Ser51. Recent studies have linked inherited mutations in any of the five eIF2B subunits to a fatal human disorder known as childhood ataxia with central nervous system hypomyelination (CACH) or vanishing white matter (VWN) disease.

Translation initiation factor eIF-2B subunit β is a part of a five-subunit complex that catalyzes guanine nucleotide exchange on eIF2. EIF2B2 is associated with the cause of autosomal recessive disorders. Autosomal recessive inherited mutations in each of the five eukaryotic initiation factor 2B (eIF2B) subunits are known to cause white matter abnormalities called eIF2B-related disorders.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)