추천 제품
Grade
Molecular Biology
for molecular biology
양식
buffered aqueous glycerol solution
분자량
28 kDa
UniProt 수납 번호
저장 온도
−20°C
유전자 정보
Escherichia coli K12 ... xthA(946254)
일반 설명
A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.
애플리케이션
Suitable for:
- Production of strand specific probes
- Preparation of single stranded templates for Sanger dideoxy sequencing
- Site directed mutagenesis
성분
Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.
단위 정의
One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.
관련 제품
제품 번호
설명
가격
Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Jan Silhan et al.
Nucleic acids research, 40(5), 2065-2075 (2011-11-10)
We have previously demonstrated that the two Exonuclease III (Xth) family members present within the obligate human pathogen Neisseria meningitidis, NApe and NExo, are important for survival under conditions of oxidative stress. Of these, only NApe possesses AP endonuclease activity
Hui Chen et al.
Chemical communications (Cambridge, England), 48(2), 269-271 (2011-11-23)
We describe herein a novel exonuclease III aided amplification method based on single walled carbon nanotube quenching (EASQ) for sensitive and convenient nucleic acid detection, which enabled 80-fold decrease of detection limit for HIV1 DNA assay compared with no target
Xu-Hua Zhao et al.
Analytica chimica acta, 727, 67-70 (2012-05-01)
Based on the super fluorescence quenching efficiency of graphene oxide and exonuclease III aided signal amplification, we develop a facile, sensitive, rapid and cost-effective method for DNA detection. In the presence of target DNA, the target-probe hybridization forms a double-stranded
Lu Peng et al.
Biosensors & bioelectronics, 35(1), 475-478 (2012-04-03)
We have developed a novel DNA assay based on exonuclease III (ExoIII)-induced target recycling and the fluorescence quenching ability of graphene oxide (GO). This assay consists of a linear DNA probe labeled with a fluorophore in the middle. Introduction of
Feng Xuan et al.
Analytical chemistry, 84(12), 5216-5220 (2012-06-23)
Taking advantage of the preferential exodeoxyribonuclease activity of exonuclease III in combination with the difference in diffusivity between an oligonucleotide and a mononucleotide toward a negatively charged ITO electrode, a highly sensitive and selective electrochemical molecular beacon (eMB)-based DNA sensor
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