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Merck
모든 사진(2)

문서

D5652

Sigma-Aldrich

Dulbecco′s Phosphate Buffered Saline

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

동의어(들):

DPBS

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About This Item

MDL number:
UNSPSC 코드:
12352207
eCl@ss:
32129211
NACRES:
NA.25

Quality Level

형태

powder

기술

cell culture | mammalian: suitable

pH

7.2-7.8

solubility

water: 9.6 g/L, clear

저장 온도

2-8°C

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일반 설명

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

애플리케이션

D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
  • to dissolve Angelica gigas powder for microencapsulation
  • to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
  • to wash amniotic membrane for cellular analyses

생화학적/생리학적 작용

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

수량

Formulated to contain 9.6 grams of powder per liter of medium.

기타 정보

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Zhaoxia Zhang et al.
Molecular vision, 24, 434-442 (2018-07-24)
Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the RGR gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression
Ana C Bohórquez et al.
Journal of colloid and interface science, 506, 393-402 (2017-07-28)
The rotational diffusion of polyethylene glycol coated magnetic nanoparticles in serum albumin solutions was investigated in a range spanning 0mgmL
Functional enhancement of ultrafine Angelica gigas powder by spray-drying microencapsulation
Choi KO, et al.
LWT--Food Science and Technology, 101, 161-166 (2019)
Lauren E Prusinski Fernung et al.
The American journal of pathology, 188(10), 2293-2306 (2018-08-04)
Uterine fibroids (UFs) are clonal, hormonally regulated, benign smooth-muscle myometrial tumors that severely affect female reproductive health, although their unknown etiology limits effective care. UFs occur fourfold more commonly in African American women than in Caucasian women, and African American
Iqbal Hyder et al.
BMC molecular and cell biology, 21(1), 9-9 (2020-03-01)
Gene transfer by electroporation is an established method for the non-viral mediated transfection of mammalian cells. Primary cells pose a particular challenge for electroporation-mediated gene transfer, since they are more vulnerable than immortalized cells, and have a limited proliferative capacity.

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