LB198

98062320, human blood, Lymphoblastoid

• UNSPSC 코드: 41106514

속성

• product name: LB198, 98062320
• 생물학적 소스: human blood
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Lymphoblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human lymphocytes, Trichothiodystrophy heterozygote, EBV-transformed

세포주 설명

LB198 has been derived from mononuclear cells from peripheral blood of a 45 year old female. The cells have been transformed with EBV. The patient has been shown to be heterozygote for trichothiodystrophy (TTD) and is the parent of LB199 (Sigma Catalogue number.98062321) TTD is a rare genetic disorder with deficiency in excision repair of UV damage. Fibroblasts from the same individual have been designated TTD HM 1BR.

애플리케이션

DNA repair deficiency studies

배양 배지

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

기타 정보

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