MOR/0.4R

96042334, human lung, Not specified

• UNSPSC 코드: 41106514

속성

• product name: MOR/0.4R, 96042334
• 생물학적 소스: human lung
• 성장 모드: Semi-adherent aggregates
• 핵형: Not specified
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human lung adenocarcinoma, drug-resistant

세포주 설명

MOR/0.4R has been developed from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). This drug-resistant cell line accumulates lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance-associated protein (MRP). The expression of a 190kDa membrane protein associated with the degree of drug resistance has been indicated. Cells grow as easily detaching aggregates

애플리케이션

Analysis of acquired drug resistance, MRP studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 7
TPOX: 11
vWA: 15

배양 배지

RPMI 1640 + 2mM Glutamine + 0.4μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

기타 정보

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