NCI-H69/LX20

96042332, human lung, Not specified

• UNSPSC 코드: 41106514

속성

• product name: NCI-H69/LX20, 96042332
• 생물학적 소스: human lung
• 성장 모드: Aggregates in suspension
• 핵형: Not specified
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian small cell lung carcinoma

세포주 설명

The drug-resistant cell line NCI-H69/LX20 has been derived by exposure of the parent line, H69 (ECACC Number 91091802) to doxorubicin, also known as adriamycin. These multi drug resistant (MDR) cells have been found to be recognised by various anti-P-glycoprotein antibodies.

애플리케이션

Analysis of acquired drug resistance, study of small cell lung carcinoma, MDR studies.

배양 배지

RPMI 1640 + 2mM Glutamine + 2μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). ECACC has found that the following supplement will assist good growth of cells: sodium selenite 10nM, Insulin 250ng/ml, Transferrin 10ug/ml and sodium pyruvate 1mM.

계대배양 정규 작업

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. It is recommended to culture the cells without drug upon resuscitation until the first passage. Resuscitate into 9 ml of medium. Immediately after resuscitation perform a centrifugation ste

기타 정보

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