RENT tsS1.1

92092515, rat endometrium, Cuboidal

• UNSPSC 코드: 41106514

속성

• 제품명: RENT tsS1.1, 92092515
• 생물학적 소스: rat endometrium
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Cuboidal
• 제품: Not specified
• 수용체: Glucocorticoid
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed

세포주 설명

Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. The cells carry vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43 markers. The transforming agent is a ts mutant of SV 40 T-antigen (tsA58).

배양 배지

DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.

기타 정보

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