TT

92050721, human thyroid, Not specified

• UNSPSC 코드: 41106514

속성

• product name: TT, 92050721
• 생물학적 소스: human thyroid
• 성장 모드: Adherent
• 핵형: Aneuploid karyotype with marker chromosomes
• 형태학: Not specified
• 제품: Calcitonin, Carcinoembryonic antigen (CEA)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian thyroid medullary carcinoma

세포주 설명

Established from a thyroid medullary carcinoma of a 77 year old Caucasian female. Tumourigenic in nude mice.

애플리케이션

Tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18

배양 배지

Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Population doubling is very slow, cells can take up to 5 days to reach 50% confluency; media change 2-3 times a week is recommen

기타 정보

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