NCTC 2071

90050803, mouse connective tissue, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: NCTC 2071, 90050803
• 생물학적 소스: mouse connective tissue
• 성장 모드: Adherent
• 핵형: Modal no. 63
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse C3H/An connective tissue

세포주 설명

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

애플리케이션

Nutritional, immunological and toxicological studies

배양 배지

NCTC 135 100% + 2mM Glutamine.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

기타 정보

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