생물학적 소스
Mouse + Human Transfectoma
ImmunoDon
Not applicable
골수종
J558L
반응성
Anti-NP (4-hydroxy-3 nitrophenacetyl)
기술
cell culture | mammalian: suitable
동형
IgM
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Mouse J558L + Mouse VH gene + Human C(μ) gene
세포주 설명
This human chimeric antibody is specifically directed against the hapten-4-hydroxy-3-nitrophenacetyl. V lambda of the endogenous light chain of plasmacytoma line complements VNP of transfected heavy chain to yield specific antibody. Gene marker: gpt.
배양 배지
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
계대배양 정규 작업
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
기타 정보
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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