추천 제품
product name
ONE58 cell line human, 10092313
생물학적 소스
human esophagus
설명
Human malignant mesothelioma
성장 모드
Adherent
핵형
Not specified
형태학
Epithelial-like: cells are spindle-shaped with few vacuoles.
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
cancer
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human malignant mesothelioma
세포주 설명
This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. ONE58 is tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0143
애플리케이션
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
배양 배지
RPMI 1640 (with 2mM L-Glutamine+25mM HEPES) + 5% FCS
계대배양 정규 작업
Optimal split ratio 1:4 (seeding density 1.2 x 104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.
기타 정보
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia
시험 성적서(COA)
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