JU77 cell line human

10092309, human lung, epithelioid: spindly with few vacuoles. Cobblestone morphology at confluency.

• UNSPSC 코드: 41106514

속성

• product name: JU77 cell line human, 10092309
• 생물학적 소스: human lung
• 설명: Malignant mesothelioma cells from human lung
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human malignant mesothelioma

세포주 설명

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140

애플리케이션

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

배양 배지

RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

계대배양 정규 작업

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm²).Cells incubated at 37°C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

기타 정보

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Depositor and originator: Richard Lake - University of Western Australia, Australia