108CC5-BU-5

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Mouse x Rat Hybridoma nerve
• 포장: tube of 5 μg 08062567-DNA-5UG; pkg of vial of cells 08062567-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Neuronal
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse neuroblastoma x Rat glioma hybrid

세포주 설명

A bromodeoxyuridine resistant mutant of 108CC5 (Sigma Catalogue number 08062521), a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid

애플리케이션

Used in the generation of cell hybrids.

배양 배지

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine. The cells can be grown in the absence of bromodeoxyuridine

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. The cultures rapidly produce lactic acid through anaerobic glycolysis causing the media to become acidified. The medium must never be allowed to become acidic as cells detach in clumps and take many passages to recover. Frequent inspection of cultures is therefore required. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell pellet in fresh medium using the appropriate volume to achieve the correct seeding density.

기타 정보

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