추천 제품
product name
VP229, 05092804
생물학적 소스
human breast
성장 모드
Adherent
핵형
Mean no. 74, modal no. 66, range 55-134
형태학
Epithelial
제품
Not specified
수용체
Not specified
기술
cell culture | mammalian: suitable
관련 질환(들)
cancer
배송 상태
dry ice
저장 온도
−196°C
세포주 기원
Human Breast Cancer
세포주 설명
Derived from a breast cancer biopsy removed from a 47 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade III, tumour cells did not stain positive for oestrogen receptors. The VP229 cell line was derived from the same individual as the cell line VP267. VP229 was derived from the primary tumour and VP267 was derived 14 months later from a local recurrence of the tumour. The individual was recorded to live for 2 years after surgery performed to remove the primary tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.
배양 배지
MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.
계대배양 정규 작업
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s
기타 정보
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시험 성적서(COA)
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