CAS9RFPV

Cas9 RFP Lentiviral Particles

• UNSPSC 코드: 41106621
• NACRES: NA.51

속성

• 형태: liquid
• 포장: pkg of 8 x 25 μL (aliquots)
• 농도: ≥5 x 10 ^{8 VP/ml virus (via p24 assay)}
• 응용 분야: CRISPR
• 리포터 유전자: RFP
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Ready-to-use Cas9-RFP lentiviral particles enable immediate transduction of a wide range of cell lines. Cas9-RFP lentiviral particles efficiently and stably integrate Cas9 and RFP linked by a 2A peptide and driven by the EF1 alpha promoter (EF1a-Cas9-2A-GFP) for strong expression in both dividing and non-dividing cell lines. They are ideal for avoiding the tedious lentivirus production process and are one part of a two part CRISPR system with individual Cas9 and gRNA expression vectors. For easy fluorescence microscopy a MOI greater than 200 should be employed.

To order gRNA in any format click here

애플리케이션

Functional Genomics/Target Validation

• Creation of gene knockouts in multiple cell lines
• Complete knockout of genes not amenable to RNAi
• Creation of cell lines stably expressing Cas9-RFP

특징 및 장점

• Highly specific
• Highly active
• Ready to infect/transduce

원리

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

단위 정의

VP/ml is the concentration unit of measure for viral titer estimated by p24 assay.

법적 정보

CRISPR Label License

Lentiviral, WPRE, and Evrogen Licenses

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable