추천 제품
재조합
expressed in E. coli
Quality Level
분석
≥95% (SDS-PAGE)
형태
buffered aqueous solution
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
유전자 정보
mouse ... Bid(12122)
일반 설명
Mouse BID cleaved with caspase-8 generates the amino-terminal fragment (amino acids 1-59, 7 kDa) and the carboxy-terminal fragment (amino acids 60-195, 15 kDa). On size exclusion chromatography cleaved BID elutes at 27 kDa indicating that the fragments remain associated.
생화학적/생리학적 작용
Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.
물리적 형태
0.2 μm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.
분석 메모
Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
J Zha et al.
Science (New York, N.Y.), 290(5497), 1761-1765 (2000-12-02)
Many apoptotic molecules relocate subcellularly in cells undergoing apoptosis. The pro-apoptotic protein BID underwent posttranslational (rather than classic cotranslational) N-myristoylation when cleavage by caspase 8 caused exposure of a glycine residue. N-myristoylation enabled the targeting of a complex of p7
X Luo et al.
Cell, 94(4), 481-490 (1998-09-04)
We report here the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as Fas and TNF. Peptide mass fingerprinting identified this protein
H Li et al.
Cell, 94(4), 491-501 (1998-09-04)
We report here that BID, a BH3 domain-containing proapoptotic Bcl2 family member, is a specific proximal substrate of Casp8 in the Fas apoptotic signaling pathway. While full-length BID is localized in cytosol, truncated BID (tBID) translocates to mitochondria and thus
M C Wei et al.
Genes & development, 14(16), 2060-2071 (2000-08-19)
TNFR1/Fas engagement results in the cleavage of cytosolic BID to truncated tBID, which translocates to mitochondria. Immunodepletion and gene disruption indicate BID is required for cytochrome c release. Surprisingly, the three-dimensional structure of this BH3 domain-only molecule revealed two hydrophobic
BCL-2 family members and the mitochondria in apoptosis.
A Gross et al.
Genes & development, 13(15), 1899-1911 (1999-08-13)
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.