C3874

Cytochrome P450 Microsome Preparation human

2C9 Isozyme, recombinant, expressed in Saccharomyces cerevisiae, Coexpressed with human NADPH reductase and human cytochrome b₅

• 동의어(들): Cerosomes
• 효소 위원회 번호: 1.14.14.1 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: human
• 재조합: expressed in Saccharomyces cerevisiae
• 양식: solution
• 특이 활성도: 2.0-3.0 units/pmol enzyme
• secondary activity: 1300-1650 units/mg protein (Reductase activity)
• 분자량: 45-60 kDa
• UniProt 수납 번호: P11712
• 응용 분야: cell analysis
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

애플리케이션

Cytochrome P450 Microsome Preparation Human is recombinant and expressed in S. cervisiae. It contains human NADPH Reductase and cytochrome b⁵. It is used to catalyze the oxidative metabolism of xenobiotics and to study the biotransformation and elimination of drugs and pollutants from the body. Cytochrome P450 is used to study hormone synthesis and breakdown, cholesterol synthesis, drug metabolism and bioactivation and vitamin D metabolism[1].

생화학적/생리학적 작용

Microsomal cytochrome P450 enzymes are found primarily in the endoplasmic reticulum of liver tissue. They catalyze the oxidative metabolism of xenobiotics and are the major enzymes involved in drug metabolism and bioactivation[1]. The reactions are achieved through a mixed monooxygenase system. The cytochrome P450 enzymes range in molecular mass between 45-60 kDa.

단위 정의

P450 Activity: One unit will oxidize 1.0 picomole of 7-benzoxymethyloxy-3-cyanocoumarin per minute at pH 7.4 at 37 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome c per minute in the presence of NADPH at pH 7.8 at 25 °C.

물리적 형태

Supplied as a solution containing 50 mM potassium phosphate, pH 7.4, 5% glycerol, 1 mM EDTA, 1 mM PMSF, and 2 mM DTT

분석 메모

CYP450 content: 50.0-65.0 picomoles per mg protein

기타 정보

For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.