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Merck
모든 사진(1)

문서

C-14098

Sigma-Aldrich

Human Mononuclear Cells (hMNC)

Isolated from peripheral blood, single donor, 1 million cells per pellet

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About This Item

UNSPSC 코드:
41106514
NACRES:
NA.81

생물학적 소스

human peripheral blood

포장

pkg of 1,000,000 cells

형태학

(mononuclear)

응용 분야

cell analysis (DNA, RNA, and protein)

배송 상태

dry ice

저장 온도

−20°C

관련 카테고리

일반 설명

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

세포주 기원

Blood

애플리케이션

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from umbilical cord blood or peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

품질

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

경고

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

물리적 형태

Pellet is dissolved in RNAlater® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

계대배양 정규 작업

Click here for more information.

기타 정보

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

법적 정보

RNAlater is a registered trademark of Ambion, Inc.

면책조항

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

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