추천 제품
생물학적 소스
mouse
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
14H5, monoclonal
양식
buffered aqueous solution
종 반응성
human
농도
2 mg/mL
기술
immunocytochemistry: 4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: suitable
동형
IgG1
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... BUB1(699)
일반 설명
BUB1 is spindle checkpoint kinase that modulates kinetochore-independent segregation of chromosomes and cell proliferation. This kinase regulates the phosphorylation of MAD1 and itself. As a result, BUB1 mediates chromosome-spindle attachments during mitosis. BUB1 also phosphorylates Cdc20 which subsequently facilitates accurate checkpoint signaling . Monoclonal Anti-BUB1 antibody is specific for human BUB1 (approx. 150 kDa).
면역원
recombinant human BUB1.
애플리케이션
Monoclonal Anti-BUB1 antibody is suitable for use in western blot, indirect ELISA, microarray, immunocytochemistry (4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol) and immunoprecipitation.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
B Ouyang et al.
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 9(10), 877-885 (1998-10-28)
Eukaryotic cells have evolved a mechanism that delays the onset of anaphase until chromosomes are properly positioned on the spindle. To understand the molecular basis of such surveillance mechanism in human cells, we have cloned a full-length cDNA encoding a
Zhanyun Tang et al.
Molecular cell, 16(3), 387-397 (2004-11-05)
To ensure the fidelity of chromosome segregation, the spindle checkpoint blocks the ubiquitin ligase activity of APC/C(Cdc20) in response to a single chromatid not properly attached to the mitotic spindle. Here we show that HeLa cells depleted for Bub1 by
T W Seeley et al.
Biochemical and biophysical research communications, 257(2), 589-595 (1999-04-13)
The BUB/MAD signaling pathway monitors attachment of chromosomes to spindle poles in mitotic cells. Mutations of the human BUB1 locus were identified in cancer cells exhibiting an unstable chromosomal complement. We report that the human BUB3 gene maps to a
Christiane Klebig et al.
The Journal of cell biology, 185(5), 841-858 (2009-06-03)
The kinetochore-bound protein kinase Bub1 performs two crucial functions during mitosis: it is essential for spindle checkpoint signaling and for correct chromosome alignment. Interestingly, Bub1 mutations are found in cancer tissues and cancer cell lines. Using an isogenic RNA interference
Min Wu et al.
Nature communications, 10(1), 273-273 (2019-01-19)
Faithful chromosome segregation requires proper chromosome congression at prometaphase and dynamic maintenance of the aligned chromosomes at metaphase. Chromosome missegregation can result in aneuploidy, birth defects and cancer. The kinetochore-bound KMN network and the kinesin motor CENP-E are critical for
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