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Merck
모든 사진(1)

문서

A8311

Sigma-Aldrich

Antifoam 204

aqueous emulsion for bacterial and mammalian systems

동의어(들):

organic antifoam

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About This Item

MDL number:
UNSPSC 코드:
12161901
NACRES:
NA.85

Quality Level

형태

emulsion (aqueous)

농도

100% (organic)

density

1.01 g/mL at 25 °C

응용 분야

microbiology

저장 온도

room temp

적합성

(Mammalian (suspension))
bacteria (fermentation)

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일반 설명

Antifoam 204 is an organic antifoam used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.

애플리케이션

Suitable for preventing or minimizing foaming in microbial fermentation systems and mammalian suspension cultures.
Antifoam 204 has been used:
  • in the expression and purification of PUL (PLAP, Ufd3p, and Lub1p) domain
  • in fed-batch fermentation
  • to prevent bubble formation due in aeration in Luria Bertani (LB) culture medium for bacterial growth
  • in the batch fermentation with mineral medium

성분

Antifoam 204 is a 100% active components and a mixture of non-silicone organic defoamers in a polyol dispersion.

기타 정보

Contains 100% active components and is a mixture of non-silicone organic defoamers in a polyol dispersion. Can be sterilized repeatedly.

제조 메모

For use in microbiological media Sigma recommends a starting concentration of between 0.005% and 0.01%.
The optimal amount of antifoam required for various applications will need to be determined. Antifoam 204 is soluble in methanol, ethanol, toluene, xylene, perchloroethylene, and cold water at temperatures below 15 °C. It is insoluble in warm water and ethylene glycol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


시험 성적서(COA)

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Biotechnology progress, 23(4), 767-784 (2007-06-15)
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