추천 제품
생물학적 소스
rabbit
결합
unconjugated
항체 형태
whole antiserum
항체 생산 유형
primary antibodies
클론
polyclonal
포함
15 mM sodium azide
종 반응성
mouse, rat, human, chicken
기술
indirect immunofluorescence: 1:40 using cultured chicken fibroblasts
western blot: suitable using rabbit skeletal muscle α-actin1
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
유전자 정보
human ... ACTA1(58)
mouse ... Acta1(11459)
rat ... Acta1(29437)
일반 설명
Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the func tional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two γ actins (γ smooth muscle and γ non-muscle). Difficulties have been encountered in producing polyclonal antibodies due to the highly conserved nature of actin.
Rabbit Anti-Actin reacts with pure actin by counter immunoelectrophoresis. The product also stains the I-bands of stretched skeletal muscle.
Rabbit polyclonal anti-Actin antibody reacts with rat, chicken, human, and mouse actin. By immunoblotting, cross-reactivity with other proteins may be observed.
면역원
actin from chicken back muscle
애플리케이션
May be used for studying the intracellular distribution of actin structures and their functions in the contractile system.
Rabbit anti-actin antibody has been used for western blot assays using mouse lung homogenates and human umbilical artery endothelial cell (HUAEC) lysates.
Rabbit anti-actin antibody was used to visualize actin in human sperm smears. Samples were incubated with the antibody (1:80) for 2 hours at 37 degrees.
Fluorescent microscopy was performed on bovine fallopian tube epithilial cells to visualize actin aggregates following inoculation with c. parvum sporozites using the rabbit anti-actin antibody from Sigma.
Fluorescent microscopy was performed on bovine fallopian tube epithilial cells to visualize actin aggregates following inoculation with c. parvum sporozites using the rabbit anti-actin antibody from Sigma.
Rabbit polyclonal anti-Actin antibody is used to tag actin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of actin in stress fibers and I-bands of skeletal muscle cells.
물리적 형태
Rabbit Anti-Actin is supplied as a liquid containing 0.1% sodium azide as preservative.
기타 정보
To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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