94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• 동의어(들): Urate oxidase
• CAS Number: 9002-12-4
• 효소 위원회 번호: 1.7.3.3 (BRENDA, IUBMB)
• MDL number: MFCD00082126
• UNSPSC 코드: 12352204
• PubChem Substance ID: 57653596
• NACRES: NA.54

속성

• 생물학적 소스: Bacillus sp. (B. fastidiosus)
• 형태: powder
• 품질: lyophilized
• 특이 활성도: ~9 U/mg
• 저장 조건: dry at room temperature
• 농도: 90—100%
• 색상: white to faint beige
• solubility: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• 응용 분야: detection
• 저장 온도: −20°C
• SMILES string: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChI key: VPXDHZMLJOJKOX-UHFFFAOYSA-N

설명

일반 설명

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

애플리케이션

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

생화학적/생리학적 작용

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

단위 정의

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

기타 정보

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H319,H334,H360FD
• 예방조치 성명서: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)